The Art of Frozen
This article describes a system used for embedding of tissues for the preparation of frozen sections. This novel system uses simple techniques and apparatus to accomplish face-down embedding in freezing-temperature steel wells.
Process of Fixation and the Nature of Fixatives
Fixation is a critical step in the preparation of histological sections. If it is not carried out under optimal conditions or if fixation is delayed, a tissue specimen can be irreversibly damaged. No matter how much care is subsequently taken in tissue...
Knife Angle in Microtomy
The correct knife angle is the subject of much misunderstanding, misleading experience, and incorrect information passed between microtomists, but in fact can be logically derived.
When to use a Vibrating Microtome for Tissue Sectioning
As the leading manufacturer of each of the major types of microtomes, Cryostats, sliding microtomes, rotary microtomes, and vibrating blade microtomes, Leica frequently fields questions as to which one is needed for a given application.
The Basic Chemistry of Hematoxylin
This article is a review of the current state of understanding of the chemistry of aluminum based hematoxylin stains.
An Introduction to Routine and Special Staining
Routine (or H&E) and special stains allow us to visualize otherwise transparent tissue under a microscope and are critical for tissue-based diagnosis.
Special Stain Techniques for the Evaluation of Mucins
Special stains that are used for the evaluation of mucins, mucin-like molecules and other carbohydrate containing macromolecules remain in demand and are utilized frequently in the histology laboratory.
Special Stains – Which One, Why and How? Part I: Mucins and Glycogen
“Special stain” is a term used to refer to many alternative staining techniques that are used when the traditional H&E does not provide all the information the pathologist or researcher needs from a tissue slide.
Special Stains – Which One, How and Why? Part II: Connective Tissue
"Special stain" is a term used to refer to many alternative staining techniques that are used when the traditional H&E does not provide all the information the pathologist or researcher needs from a tissue slide.
Special Stains – Which One, Why and How? Part III: Microorganisms – Bacteria and Fungi
Microorganisms are living organisms which include bacteria, fungi, protozoa and viruses. Bacteria, fungi and protozoa can be identified and classified with histochemical procedures and viruses are generally identified with immunohistochemical procedures.